Process for amplifying, detecting, and/or cloning nucleic acid s

Details Process for amplifying, detecting, and/or cloning nucleic acid s Process for amplifying, detecting, and/or cloning nucleic acid s

1987-06-17

1990-10-23

Martinell, James

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 691, 435 91, 4351723, 935 17, 935 78, C12Q 168, C12P 2100, C12P 1934, C12N 1500

Patent

active

049651889

ABSTRACT:
A process for amplifying any target nucleic acid sequence contained in a nucleic acid or mixture thereof comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers and extending the primers with a thermostable enzyme to form complementary primer extension products which act as templates for synthesizing the desired nucleic acid sequence. The amplified sequence can be readily detected. The steps of the reaction can be repeated as often as desired and involve temperature cycling to effect hybridization, promotion of activity of the enzyme, and denaturation of the hybrids formed.

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