Process for amplifying a target polynucleotide sequence using a

Details Process for amplifying a target polynucleotide sequence using a Process for amplifying a target polynucleotide sequence using a

1992-10-05

1996-08-13

Fleisher, Mindy

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 9121, 435 9151, C12P 1934, C12Q 168

Patent

active

055455220

ABSTRACT:
This invention relates to the use of promoters for ribonucleic acid amplification and other genetic manipulations. Processes are provided wherein complementary deoxyribonucleic acid (cDNA) is synthesized from a ribonucleic acid (RNA) sequence using a complementary primer linked to an RNA polymerase promoter region complement and then anti-sense RNA (aRNA) is transcribed from the cDNA by introducing an RNA polymerase capable of binding to the promoter region. Additional processes using the resulting aRNA are also described.

REFERENCES:
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Feinberg et al, Analytical Biochemistry 132: 6-13 (1983).
Sargent, Methods in Enzymology V 152 Academic Press Inc NY NY (1987) pp. 423-433.
Frohman, et al., PNAS 85: 8998-9002 (1988).
Eberwine, et al., Neuroscience Short Course I (Soc. for Neuroscience) 69-81 (1988).
Sarkar, et al., Science 244: 331-334 (1989).
Stoflet, et al., Science 239: 491-494 (1988).

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