Process for aligning macromolecules on a surface by passage thro

Details Process for aligning macromolecules on a surface by passage thro Process for aligning macromolecules on a surface by passage thro

1998-03-23

2000-04-25

Naff, David M.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

435 6, 435 71, 435174, 435176, 436524, 530810, 530811, 536 221, 536 254, 536124, 536127, G01N 33543, C12N 1116, C07K 1700, C07H 2100, C12Q 168

Patent

active

060543272

ABSTRACT:
Macromolecules such as nucleic acids, proteins, lipids or polysaccharides are aligned on a support surface by passing the macromolecules through a meniscus of a solvent containing the macromolecules. The meniscus may be that of a solvent between two surfaces at an interface of the solvent with air. One end of a macromolecule is attached to one surface which may be a glass surface and another end is free. The meniscus is moved relative to the surface to which the end is attached such as by evaporating the solvent or by moving the surface. As the macromolecule passes through the meniscus, the macromolecule aligns on the surface perpendicular to the meniscus. This method may be used in assaying, measuring intramolecular distance and/or separating of macromolecules.

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A. Bensimon et al., "Alignment and Sensitive Detection . . . ", Science, 265 (5181) 2096-2098 (1994).

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