Process for aligning, adhering and stretching nucleic acid stran

Details Process for aligning, adhering and stretching nucleic acid stran Process for aligning, adhering and stretching nucleic acid stran

1995-06-06

1998-11-24

Naff, David M.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

435 6, 4351721, 435174, 435176, 436518, 436524, 530810, 530811, 536 254, 536124, 536127, 935 77, 935 78, 935 19, C07H 2100, C12N 1500, C12Q 168

Patent

active

058408622

ABSTRACT:
Macromolecules such as nucleic acid strands are aligned, adhered and stretched on a support surface by passing the strands through a meniscus of a solvent containing the strands. The meniscus may be that of a solvent between two surfaces at an interface of the solvent with air. One end of a nucleic acid strand is attached to one surface which may be a glass surface and another end is free. The meniscus is moved relative to the surface to which the end is attached such as by evaporating the solvent or by moving the surface. As the nucleic acid strand passes through the meniscus, the strand elongates and aligns perpendicular to the meniscus on the surface. This method may be used in assaying, measuring intramolecular distance of and/or separating macromolecules.

REFERENCES:
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patent: 5102798 (1992-04-01), Guiseppi-Elie
Zimmermann et al., "DNA stretching on functionalized gold surfaces," Nucleic Acids Res. 22:3 (1994), pp. 492-497.
Schwartz et al., "Ordered Restriction Maps of S. cerevisiae Chromosomes Constructed by Optical Mapping," Science 262:5130 (Oct. 1993), pp. 110-114.
Parra et al., "High resolution visual mapping of stretched DNA by fluorescent hybridization," Nature Genetics, vol. 5 (Sep. 1993), pp. 17-21.
Lund et al., "Assessment of methods for covalent binding of nucleic acids to magnetic beads, Dynabeads .TM., and the characteristics of the bound nucleic acids in hybridization reactions," Nucleic Acids Res. 16(1988), pp. 10861-10890.
Bensimon, et al., Science, Sep. 30, 1994, pp. 2096-2098.
Keller, et al., Proc. Natl. Acad. Sci., vol. 86, Jul. 1989, pp. 5356-5360.

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