Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Chemical modification or the reaction product thereof – e.g.,...
Patent
1989-10-17
1991-12-31
Draper, Garnette D.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Chemical modification or the reaction product thereof, e.g.,...
530408, 530409, 530387, 435219, 424 9464, C07K 300, C07K 1500, C07K 1528, C12N 950
Patent
active
050773925
ABSTRACT:
The present invention provides a process for the activation of gene-technologically produced, biologically active proteins expressed in prokaryotes after cell digestion by solubilization under denaturing conditions and reducing conditions and subsequent reactivation under oxidizing and renaturing conditions, wherein working is carried out at a protein concentration of 1 to 1000 .mu.g./ml. and, between the solubilization and the reactivation, a dialysis is carried out against a buffer with a pH value of from 1 to 4 containing 4 to 8 mole/liter guanidine hydrochloride or 6 to 10 mole/liter urea.
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Freedman et al., J. Biol. Chem. 241(22): 5225-5232, (1966), Chem. Abst. 113(3): 21920S, (1990).
Buchner Johannes
Lenz Helmut
Rudolph Rainer
Baker R. Keith
Boehringer Mannheim GmbH
Draper Garnette D.
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