Process and test carrier for the determination of an analyte

Details Process and test carrier for the determination of an analyte Process and test carrier for the determination of an analyte

1987-11-18

1991-08-06

Kepplinger, Esther L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

422 56, 435 71, 435 792, 435 795, 435 10, 435 18, 435174, 436501, 436518, 436528, 436529, 436536, 436537, 436540, 436547, 436823, G01N 3353, G01N 33543, G01N 33536, C12Q 134

Patent

active

050377361

ABSTRACT:
The present invention provides a process for the determination of an analyte in a body fluid, in which there are used two binding components capable of specifically binding with one another, one of the binding components being enzyme-labelled and not carrier-fixed and the other binding component being carrier-fixed. The process contains a step in which the binding components are incubated with one another so that binding reaction takes place. The amount of enzyme-labelled binding component not bound to the carrier-fixed binding component is a measure of the concentration of the analyte which is determined by allowing the labelling enzyme to act upon a substrate producing a detection signal. During the specific binding reaction, incubation is carried out simultaneously with a non-fixed substrate of the labelling enzyume which does not produce a detection signal and with a carrier-fixed substrate of the labelling enzyme which produces a detection signal, the substrate not producing a detection signal being so chosen with regard to the amount used and affinity to the labelling enzyme in relation to the amount of the substrate producing a detection signal and its affinity to the labelling enzyme and in relation to the total activity of the labelling enzyme that the enzyme-catalyzed reaction of the substrate producing a detection signal is delayed until the specific binding reaction between the binding components has substantially taken place.

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patent: 4868131 (1989-09-01), Hiratsuka
Oellerich, J. Clin. Chem. Biochem., vol. 22, 1984, pp. 895-904.

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