Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Peptide containing doai
Reexamination Certificate
2004-12-23
2008-10-21
Tsang, Cecilia J (Department: 1654)
Drug, bio-affecting and body treating compositions
Designated organic active ingredient containing
Peptide containing doai
Reexamination Certificate
active
07439222
ABSTRACT:
The invention provides methods of synthesizing peptides, involving the steps of providing a composition including a peptide fragment, wherein the peptide fragment has at least one amino acid residue and includes a base-sensitive, N-terminal protecting group; removing the base-sensitive, N-terminal protecting group from the peptide fragment using a deprotection reagent that includes a base, whereby an N-terminal functionality on the peptide fragment is deprotected; removing the base from the composition to provide a residual base content of more than 100 ppm; causing a reactive peptide fragment having a reactive C-terminus and a base-sensitive N-terminal protecting group to react with the deprotected N-terminal functionality of the peptide fragment under conditions such that the reactive peptide fragment is added to the peptide fragment; and optionally repeating the deprotection and coupling steps until a desired peptide is obtained. Also provided are methods of synthesizing peptides, wherein base is removed from the composition to a point where the composition would provide a positive chloranil test. Also provided are methods of synthesizing peptides, wherein coupling is performed in basic reaction mixtures.
REFERENCES:
patent: 4108846 (1978-08-01), Meienhofer
patent: 5126273 (1992-06-01), Sheppard et al.
patent: 5356596 (1994-10-01), Nokihara et al.
patent: 5464933 (1995-11-01), Bolognesi et al.
patent: 5656480 (1997-08-01), Wild et al.
patent: 5827825 (1998-10-01), Takei et al.
patent: 6013462 (2000-01-01), Kauvar et al.
patent: 6015881 (2000-01-01), Kang et al.
patent: 6281331 (2001-08-01), Kang et al.
patent: 6469136 (2002-10-01), Bray et al.
patent: 6479055 (2002-11-01), Bolognesi et al.
patent: 2003/0125516 (2003-07-01), Bray et al.
patent: WO 90/02605 (1990-03-01), None
patent: WO 95/12608 (1995-05-01), None
patent: WO 93/25571 (1995-09-01), None
patent: WO 98/25965 (1998-06-01), None
patent: WO 99/46283 (1999-09-01), None
patent: WO 99/48513 (1999-09-01), None
Vojkovsky. Detection of Secondary Amines on Solid Phase. Peptide Research. 1995, vol. 8., No. 4, pp. 236-237.
Kaiser et al. Color Test for Detection of Free Terminal Amino Groups in the Solid-Phase Synthesis of Peptides. Analytical Biochemistry., 1970. vol. 34, No. 2, pp. 595-598.
Riniker et al. (1993) Tetrahedron Letters 49:9307-9320.
Lloyd Williams et al. (1993) Tetrahedron Letters 49:11065-11133.
Bray, Brian L. (Jul. 2003) Nature Review 2:587-593.
Andersson et al. (2000) Biopolymers 55:227-250.
CA 132:279509 (abstract).
CA 132:276545 Meldal, M. et al (abstract).
Meldal (1994)The Susceptibility of Glycans to b-elimination in Fmoc-based O-glycopeptide synthesis, International Journal of Peptide and Protein Research, 43:529-536.
Guinn Martin R.
Hodges Lewis M.
Johnston David A.
Moorlag Hendrick
Schwindt Mark A.
Buckwalter Brian L.
Cordero Garcia Marcela M
Roche Palo Alto LLC
Tsang Cecilia J
LandOfFree
Process and systems for peptide synthesis does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Process and systems for peptide synthesis, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process and systems for peptide synthesis will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4004220