Process and reagents for the detection of a monocaternary nuclei

Details Process and reagents for the detection of a monocaternary nuclei Process and reagents for the detection of a monocaternary nuclei

1988-09-15

1991-12-03

Yarbrough, Amelia Burgess

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435259, 435270, 435803, 435810, 436 63, 436 64, 436 94, 436501, 436175, 436813, 436825, 935 4, 935 21, 935 75, C12Q 168

Patent

active

050700090

ABSTRACT:
The invention relates to a process for the in vitro detection of a determined RNA, particularly of a mRNA connected with a genetic abnormality in a biological material. This process comprises a treatment of the cells contained in that material for the sake of releasing their cellular components and of exposing the RNAs yet without denaturing other nucleic acids, and then the detection operation comprising contacting RNA sought to be detected, in presence of the other cellular components, with a nucleotidic sequence complementary of the RNA sought.

REFERENCES:
patent: 4038143 (1977-02-01), Juni
patent: 4483920 (1984-11-01), Gillespie et al.
Bresser, J. et al. Proc. Nat'l Acad. Sci. U.S.A., vol. 80, 1983, pp. 6523-6527.
Perbal, B. A Practical Guide to Molecular Cloning, Wiley-Interscience, N.Y. 1984, pp. 388,389.
Bresser, J. et al, DNA vol. 2, No. 3, Sep., 1983, pp. 243-254.

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