Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1987-10-06
1990-01-09
Warden, Robert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 11, 435 19, 435 25, 435 28, 435805, 436 71, 436810, 436824, C12Q 160 A, G01N 3368, G01N 3392 B
Patent
active
048928151
ABSTRACT:
The present invention provides a process for the specific determination of the cholesterol of the HDL fraction in the presence of the LDL fraction of serum lipoproteins. Pancreatic cholesterol esterase is used to liberate cholesterol, and the liberated cholesterol then reacts with cholesterol oxidase and oxygen to form hydrogen peroxide. The kinetics of either of hydrogen peroxide formation or oxygen consumption is measured within 2 to 15 minutes after the start of the reaction between cholesterol and the oxidase. The temperature is maintained within a range of 20.degree. C., during a predetermined time interval. Specific concentrations of reactants are maintained in the reaction solution, i.e., from 0.05 to 30 U/ml pancreatic cholesterol esterase; from 0.1 to 50 U/ml cholesterol oxidase; from 1.0 to 20 mMole/liter of a tenside of the bile acid group, and 0.1 to 10 g/liter of a non-ionic detergent. The pH is kept within a range of 5 to 9. In addition, a reagent is provided which is used for specific determination of HDL fraction cholesterol in the presence of the LDL fraction of serum lipoproteins. The reagent contains pancreatic esterase (0.05 to 30 U/ml); cholesterol oxidase (0.1 to 50 U/ml) a tenside of the bile acid grop (1.5 to 8 mMole/liter) and a non-ionic detergent (0.1 to 10 g/liter), all concentrations referring to the dilution used in the test. The reagent also contains a buffer at a pH of from 5 to 9, and a system for photometric determination of hydrogen peroxide.
REFERENCES:
patent: 4164448 (1979-08-01), Roeschlau et al.
patent: 4275151 (1981-06-01), Esders et al.
patent: 4312834 (1982-01-01), Vogel et al.
patent: 4409326 (1983-10-01), Modrovich
patent: 4544630 (1985-10-01), Ziegenhorn et al.
patent: 4746605 (1988-05-01), Kerscher et al.
Kerscher Lorenz
Pautz Brigitte
Trunk Gisela
Ziegenhorn Joachim
Boehringer Mannheim GmbH
Spiegel Carol A.
Warden Robert J.
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