Process and reagent for the specific determination of HDL choles

Details Process and reagent for the specific determination of HDL choles Process and reagent for the specific determination of HDL choles

1986-09-16

1989-07-25

Rosen, Sam

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving cholesterol

435 28, C12Q 160

Patent

active

048513350

ABSTRACT:
The present invention provides a process for the specific determination of HDL cholesterol in serum or plasma by incubation with a cholesterol detection system, containing cholesterol oxidase and cholesterol esterase in a buffered aqueous medium, and measurement of a product of the cholesterol oxidase reaction or of the oxygen consumption, wherein a sample to be tested is incubated in the presence of a salt of a bile acid or of a bile acid derivative or of dioctylsulphosuccinate, a first measurement is then carried out, subsequently a non-ionic, polyethylene oxide group-containing detergent or a secondary alkane sulphonate is added, again incubated and a second measurement is carried out, the amount of HDL cholesterol being determined from the difference between the first and second measurement.
The present invention also provides a reagent for the specific determination of HDL cholesterol in serum or plasma containing cholesterol oxidase, buffer and cholesterol esterase, wherein, referred to the final solution, it contains

REFERENCES:
patent: 4164448 (1979-08-01), Roeschlau et al.
patent: 4184921 (1980-01-01), Roeschlau et al.
patent: 4226713 (1980-10-01), Goldberg
patent: 4366244 (1982-12-01), Pascal
patent: 4414326 (1983-11-01), Goldberg
patent: 4544630 (1985-10-01), Ziegenhorn

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