Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1988-09-26
1992-09-22
Kepplinger, Esther
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
435 4, 435 14, 435 27, 435 28, 435269, 436 34, 436 95, 436174, 436175, 436904, 436 87, 436 88, 436111, C12Q 126, C12Q 154, C12Q 128
Patent
active
051496330
ABSTRACT:
Process for the specific determination of the serum fructosamine content in blood or in samples obtained from blood by reaction with an appropriate color reagent and measurement of the color change thereby brought about, in which before the color reaction sample components with a non-specific reducing action and/or causing turbidity are removed and subsequently the color reagent is added at a pH value of from 10 to 12. The sample components are removed by treatment at approximately neutral pH value with a reagent composition comprising at least one enzymatic oxidation agent, optionally together with peroxidase and/or catalase and/or lipase, as well as with at least one SH group-blocking substance. A kit for the specific determination of the serum fructosamine content in blood or samples obtained from blood, comprises said reagent composition, a rebuffering reagent with a buffer which has an alkaline pH value and a color reagent for the detection of fructosamine.
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Schellong Liselotte
Siedel Joachim
Vogt Bernd
Ziegenhorn Joachim
Boehringer Mannheim GmbH
Kepplinger Esther
Scheiner Toni R.
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