Process and reagent for the rapid quantitative determination of

Details Process and reagent for the rapid quantitative determination of Process and reagent for the rapid quantitative determination of

1976-08-11

1978-03-21

Jones, Raymond N.

Textiles: fiber preparation

1951035R, G01N 3114

Patent

active

040802639

ABSTRACT:
L-lactate is quantitatively determined by the reaction thereof with nicotinamide-adenine-dinucleotide (NAD) in the presence of lactate dehydrogenase (LDH) with the formation of reduced NAD (NADH) and pyruvate, reaction of the latter with glutamate in the presence of glutamate-pyruvate-transaminase (GPT) with the formation of alanine and .alpha.-ketoglutarate in alkaline buffered solution and measurement of the NADH formed, wherein the reaction is carried out in the presence of at least 0.4 mol/liter of carbonate or bicarbonate.

REFERENCES:
patent: 3413198 (1968-11-01), Deutsch et al.
patent: 3573171 (1971-03-01), Green et al.
patent: 3778350 (1973-12-01), Bergmeyer et al.
Bergmeyer et al., Methods of Enzymatic Analysis, vol. 3, Academic Press Inc., New York, San Francisco, London, (1974), pp. 1468-1479.
Bergmeyer, Methods of Enzymatic Analysis, Academic Press, New York and London, (1965), pp. 378-380.

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