Process and reagent for the kinetic determination of enzyme subs

Details Process and reagent for the kinetic determination of enzyme subs Process and reagent for the kinetic determination of enzyme subs

1978-10-23

1980-10-21

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving cholesterol

435 14, 435 15, 435 19, 435 25, 435 26, 435 28, 435810, C12Q 150, C12Q 154, C12Q 148, C12Q 144

Patent

active

042295270

ABSTRACT:
Kinetic determination of enzyme substrates by means of coupled reactions comprising selecting the reaction parameters so that the most specific part reaction of a reaction sequence becomes rate-determining for the whole reaction sequence wherein the rate-determining part reaction follows first or pseudo-first order kinetics.

REFERENCES:
patent: 3862009 (1975-01-01), Wahlefeld et al.
patent: 3886045 (1975-05-01), Meittini
patent: 3977944 (1976-08-01), Muller-Matthesius et al.
patent: 3979262 (1976-09-01), Hunziker
Segel, Enzyme Kinetics, John Wiley & Sons, Inc., N.Y., (1975).
Bergmeyer, "Determination of Enzyme Activities", Methods of Enzymatic Analysis, Academic Press, Inc., New York (1974) pp. 121-131.

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