Process and reagent for the enzymatic determination of sulphite

Details Process and reagent for the enzymatic determination of sulphite Process and reagent for the enzymatic determination of sulphite

1984-04-02

1987-06-30

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 28, 435810, C12Q 126, C12Q 128

Patent

active

046770593

ABSTRACT:
The present invention provides a process for the enzymatic determination of sulphite, wherein the sulphite is oxidized in a buffered solution with sulphite oxidase to give sulphate, the hydrogen peroxide thereby formed being reduced in the presence of NAD and NADH with the enzyme NADH peroxidase and the consumption of NADH being determined optically.
The present invention also provides a reagent for the enzymatic determination of sulphite, wherein it contains sulphite oxidase, NADH, NAD, NADH peroxidase and buffer.

REFERENCES:
patent: 4168205 (1979-09-01), Danninger et al.
Enzyme Nomenclature, (1973), Elsevier Scientific Publishing Company, pp. 96-97, 100-101.
Barmen, (ed) 1969, Enzyme Handbook, vol. I, p. 219.
Trends in Analytical Chemistry, Band 2, Nr. 1, Jan. 1983, Cambridge GB; N. T. Crosby "Enzymes in Food Analysis", Seiten VII, VIII.
M. Dixon, E. C. Webb, "Enzymes", 3. Auflage 3. Auflage 1979, Longman Group Ltd., London, GB, Seiten, 736-737.

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