Process and reagent for the determination of transaminase

Details Process and reagent for the determination of transaminase Process and reagent for the determination of transaminase

1984-08-20

1988-03-01

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving transferase

435 26, 435853, 435857, 435822, 435810, C12Q 152, C12Q 132, C12R 101, C12R 1225, C12R 125

Patent

active

047286040

ABSTRACT:
For the determination of the transaminasae by reaction of a corresponding amino acid with .alpha.-ketoglutaric acid to form glutamate and the .alpha.-keto acid corresponding to the amino acid used, and measurement of the .alpha.-ketonic acid formed by reduction in the presence of lactate dehydrogenase (LDH) and optionally of malate dehydrogenase (MDH) with NADH, forming NAD.sup.+ and .alpha.-oxy acid, use is made of D-LDH from lactic acid bacterium.

REFERENCES:
patent: 3527332 (1970-09-01), Deutsch
patent: 3819488 (1974-06-01), Rush et al.
Enzyme Nomenclature (1972), American Elsevier Publishing Co., Inc., N.Y., pp. 46-47.
Gawehn et al., "Chemical Abstracts", vol. 82 (1975), Abstract #28119n.
The Prokaryotes, Starr et al. (eds.), vol. II (1981), Springer-Verlag, N.Y., pp. 1626-1627 and 1671.

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