Process and reagent for the determination of blood glucose in ha

Details Process and reagent for the determination of blood glucose in ha Process and reagent for the determination of blood glucose in ha

1984-01-30

1985-11-05

Rosen, Sam

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving glucose or galactose

436 67, C12Q 154

Patent

active

045514275

ABSTRACT:
A reagent and improved process for the determination of D-glucose in body fluids by reaction with ATP in the presence of hexokinase, glucose-6-phosphate dehydrogenase, NADP, Mg.sup.++ ions and buffer and measurement of the NADPH formed. The improved process requires, before reaction with ATP, the mixing of the whole blood with a reagent which is composed of 0.01 to 0.5% weight/volume of an alkyl sulphate or sulphonate or of an alkyl-aryl sulphonate wherein the alkyl moiety is C.sub.8 to C.sub.32 and optionally contains one or more hydroxylalkylamino radicals as substituents and/or is interrupted by one or more ether oxygen atoms and the alkyl-aryl radicals having C.sub.8 to C.sub.18 alkyls and optionally a preserving agent.

REFERENCES:
patent: 4416982 (1983-11-01), Tsuda et al.
Chemical Abstracts Index Guide, (1982), p. 1285G.

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