Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1988-02-19
1990-07-31
Kepplinger, Esther M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435805, 435810, 436501, 436504, 436512, 436518, 436538, 436541, 436542, 436827, G01N 33535, G01N 3353, G01N 33563, G01N 33543
Patent
active
049450425
ABSTRACT:
The present invention provides a process for the determination of an antibody by incubation with three different reagents R.sub.1, R.sub.2 and R.sub.3, of which R.sub.1 and R.sub.3 are present in liquid phase and are bindable with the antibody, R.sub.2 is present bound to a solid phase and is bindable with R.sub.1 and R.sub.3 carries a label, separation of the solid phase from the liquid phase and measurement of the label in the solid phase, wherein as R.sub.1 there is used a conjugate of a substance specifically recognized by the antibody to be determined and a reaction component of a specific binding system, as R.sub.3 there is used a conjugate of a substance specifically recognized by the antibody to be determined and a label and as R.sub.2 there is used the other binding component of the specific binding system.
The present invention also provides a composition for the determination of an antibody, wherein it contains two different soluble reagents R.sub.1 and R.sub.3 specifically bindable with the antibody, of which R.sub.1 carries a reaction component of a specific binding system and R.sub.3 carries a label and also a receptor R.sub.2 which is present in solid phase and is bindable with the receptor R.sub.1, the insoluble receptor R.sub.2 and the soluble receptor R.sub.1 being present physically separated from one another.
REFERENCES:
patent: 4778751 (1988-10-01), El Shami et al.
Engel Wolf D.
Geiger Thomas
Boehringer Mannheim GmbH
Kepplinger Esther M.
Scheiner Toni R.
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