Process and reagent for the determination of .alpha.-amylase

Details Process and reagent for the determination of .alpha.-amylase Process and reagent for the determination of .alpha.-amylase

1984-06-28

1987-10-06

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 22, 435810, C12Q 134, C12Q 140

Patent

active

046983007

ABSTRACT:
A process and reagent are described for the determination of .alpha.-amylase by enzymatic splitting of an .alpha.-amylase substrate in the presence of .alpha.-glucosidase and .beta.-glucosidase and measurement of a fission product. The substrate utilized is a 4-nitrophenyl-.beta.,D-maltoheptaoside substituted in the phenyl ring by an electron withdrawing group, preferably a 2-chloro group.

REFERENCES:
patent: 4102747 (1978-07-01), Driscoll et al.
patent: 4145527 (1979-03-01), Burns et al.
patent: 4225672 (1980-09-01), Hall
patent: 4233403 (1980-11-01), Menson et al.
Wallenfels et al, Fresenius Z. Anal. Chem., 301, 169 (1980).

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