Process and reagent for the determination of .alpha.-amylase

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving glucose or galactose

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435 15, 435 22, 435 18, 435 26, 435810, 435805, C12Q 154, C12Q 148, C12Q 140, C12Q 134, C12Q 132

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045446318

ABSTRACT:
.alpha.-Amylase is determined by the enzymatic splitting of an .alpha.-amylase substrate and measurement of a fission product, wherein there is used as a substrate a maltoheptaose compound of the formula ##STR1## wherein R is a glucoside, phenylglucoside, mononitrophenylglucoside, dinitrophenylglucoside, sorbitol or gluconic acid group. Reagents comprising such a substrate and a system for the determination of a fission product formed from the amylase substrate by .alpha.-amylase, are also provided.

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patent: 4237221 (1980-12-01), Gauhl et al.

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