Process and reagent for the determination of .alpha.-amylase

Details Process and reagent for the determination of .alpha.-amylase Process and reagent for the determination of .alpha.-amylase

1987-05-15

1990-07-31

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 22, 435 7021, 4351722, 4352402, 435188, 435810, 436537, 436548, C12Q 140, G01N 3353

Patent

active

049450433

ABSTRACT:
The present invention provides a process for the determination of .alpha.-amylase by cleavage of a substrate in the presence of one or more auxiliary enzymes and measurement of a cleavage product, wherein the reaction is carried out in the presence of monoclonal antibodies against .alpha.-amylase.
The present invention also provides a reagent for the determination of .alpha.-amylase containing a substrate, one or more auxiliary enzymes and a system for the measurement of a cleavage product, wherein it also contains at least one monoclonal antibody against .alpha.-amylase.

REFERENCES:
patent: 4446233 (1984-05-01), Hargreaves et al.
patent: 4493890 (1985-01-01), Morris
patent: 4544631 (1985-10-01), Rauscher
patent: 4649108 (1987-03-01), Blair
Tietz, N. (ed), Textbook of Clinical Chemistry, pp. 725-735 (1986).

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