Process and reagent for the determination of a specifically bind

Details Process and reagent for the determination of a specifically bind Process and reagent for the determination of a specifically bind

1989-07-05

1999-01-12

Scheiner, Toni R.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

Carrier is organic

436501, 436536, 436518, 436534, 436539, 435 725, 435 75, 435 792, 435 793, G01N 3353

Patent

active

058588035

ABSTRACT:
The present invention provides a process for the determination of a specifically bindable substance by incubation of the sample solution with at least three receptors R.sub.1, R.sub.2 and R.sub.3, of which R.sub.1 and R.sub.2 are bindable with one another and R.sub.3 is specifically bindable with the substance to be determined and measurement of the agglutination which takes place in the case of the reaction, wherein, as receptor R.sub.1, there is used a conjugate of a partner of a specifically binding pair P and of a substance S which corresponds to the substance to be determined or is a derivative thereof and has at least one epitope of the substance to be determined, as receptor R.sub.2 there is used a receptor which has at least two binding positions for P and as receptor R.sub.3 there is used a receptor which has at least two binding positions, of which at least one binds specifically with an epitope of the substance to be determined or of S. The present invention also provides a reagent for carrying out the above process.

REFERENCES:
patent: 4506009 (1985-03-01), Lenhoff et al.
patent: 4829011 (1989-05-01), Gibbons
patent: 5108896 (1992-04-01), Philo et al.

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