Process and reagent for the determination of a reaction partner

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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436512, 436518, 436523, 436531, 436532, 436533, 436540, 436809, 436819, 436825, 436826, G01N 33543, G01N 33547, G01N 33535

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048807311

ABSTRACT:
For the determination of a reaction partner of an immunological reaction according to the principle of immunoassay, the reaction partner to be determined is brought into contact with a marked specific receptor R.sub.1 and at least 1 unmarked receptor R.sub.2, one of the unmarked receptors R.sub.2 being bonded on a solid phase by a binding-capable substance R.sub.3. In order to determine the sample blank value, the unmarked receptor R.sub.2, which is bonded by the receptor fixed on the solid phase, is then replaced by another unmarked receptor R'.sub.2 which does not react with the reaction partner of R.sub.2 to be determined.

REFERENCES:
patent: 4320109 (1982-03-01), Wolf et al.
patent: 4467031 (1984-08-01), Gallati et al.
patent: 4535057 (1985-08-01), Dreesman et al.
patent: 4729961 (1988-03-01), Avrameas et al.
patent: 4791056 (1988-12-01), Sizto et al.

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