Process and reagent for specific determination of pancreatic alp

Details Process and reagent for specific determination of pancreatic alp Process and reagent for specific determination of pancreatic alp

1991-09-19

1996-11-12

Nucker, Christine M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 7021, 4351722, 43524027, 435810, 436548, 53038826, 935110, G01N 33573, C07K 1640

Patent

active

055739180

ABSTRACT:
The present invention provides a process for the specific determination of pancreatic alpha-amylase in the presence of salivary alpha-amylase in body fluids, especially in serum, plasma, duodenal juice or urine, by reaction with a system for the detection of alpha-amylase in the presence of a monoclonal antibody which inhibits salivary alpha-amylase wherein, as inhibitor, there is used a first monoclonal antibody which specifically inhibits the salivary enzyme by less than 97%, in combination with a second monoclonal anti-saliva alpha-amylase antibody which inhibits this enzyme by less than 10%.
The present invention also provides a reagent for the specific determination of pancreatic alpha-amylase in the presence of salivary alpha-amylase in body fluids, especially in serum, duodenal juice, plasma or urine, containing a system for the detection of alpha-amylase and a monoclonal antibody against salivary alpha-amylase wherein it contains a first monoclonal antibody which specifically inhibits the salivary enzyme by less than 97%, in combination with a second monoclonal anti-salivary alpha-amylase antibody which inhibits this enzyme by less than 10%.

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