Process and reagent for increasing the quantum yield in chemilum

Details Process and reagent for increasing the quantum yield in chemilum Process and reagent for increasing the quantum yield in chemilum

1986-12-10

1989-05-30

Thexton, Matthew A.

Compositions

Chemiluminescent

436513, 536 27, C09K 1100

Patent

active

048349186

ABSTRACT:
The present invention provides a process for increasing the quantum yield in the case of the oxidation of luminol or of a 7-dialkylaminonaphthalene-1,2-dicarboxylic acid hydrazide, each alkyl radical of which contains up to 3 carbon atoms, by a peroxide compound in the presence of peroxidase ( POD), wherein the reaction is carried out in the presence of fluorescein, the concentration of the fluorescein being in a concentration range which gives a quantum yield which is greater than the sum of the quantum yields of the individual chemiluminescing materials.
The present invention also provides a reagent for the determination of POD, wherein it contains luminol or a 7-dialkylaminonaphthalene-1,2-dicarboxylic acid hydrazide, each alkyl radical of which contains up to 3 carbon atoms, fluorescein, a hydrogen peroxide provider, a buffer substance (pH 7.5 to 9) and optionally a sequestering agent.

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