Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1992-03-17
1993-10-05
Kepplinger, Esther L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
435 24, 435 4, 435803, 435188, 436174, 436177, C12Q 137, C12Q 100, G01N 3000
Patent
active
052504182
ABSTRACT:
The present invention provides a process for the determination of the activity of chymotrypsin or trypsin in feces by measurement of the rate of fission of an appropriate substrate by a fecal suspension in an aqueous or aqueous-organic medium, wherein a fecal sample is suspended in the presence of a surface-active agent.
The present invention also provides a reagent for carrying out this process, comprising a surface-active agent, an enzyme substrate and an aqueous salt solution, said reagent having a pH value of from 7 to 11.
REFERENCES:
patent: 4282001 (1981-08-01), Klose et al.
Haverback, B., et al. "Measurement of Trypsin and Chymotrypsin in Stool", Gastroenterology, 44, pp. 588-597 (1963).
Del Mar, E. G., et al., "A Sensitive New Substrate for Chymotrypsin," Analytical Biochemistry, 99, pp. 316-320 (1979).
Kaspar, P., et al., "A New Photometric Method for the Determination of Chymotrypsin in stool," Fresenius Z Anal Chem, 311 (1982) 391-92.
Goldberg, D. M., et al., "Binding of trypsin and chymotrypsin by human intestinal mucosa," Biochim. Biophys. Acta., 167, pp. 613-615 (1968).
Remtulla et al., "Is Chymotrypsin Output a Better Diagnostic Index than the Measurement of Chymotrypsin in Random Stool?", Enzyme 39, 190-8 (1988).
Goldberg et al.; "Fate of Trypsin and Chymotrypsin in the Human Small Intestine", Gut 10, 477-83 (1969).
Kaspar et al., "New Photometric Assay for Chymotrypsin in Stool," Clin. Chem. 30, 1753-7 (1984).
Kaspar et al., "The Distribution of Chymotripsin within the Feces and Description of a New Device for the Preparation of Stool Samples", Clin. Chem. 30, 1864-6 (1984).
Remtulla et al., "Stool Chymotrypsin Activity Measured by a Spectrophotometric Procedure to Identify Pancreatic Disease in Infants", Clin. Biochem. 19, 341-7 (1986).
Durie et al., "Biochemical Tests of Pancreatic Function in Infancy and Childhood", Adv. Clin. Enzymol. 4, 77-92 (1986).
Kaspar Klaus P.
Moller Gerald
Boehringer Mannheim GmbH
Kepplinger Esther L.
Scheiner Toni R.
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