Process and reagent composition for determination of fructosamin

Details Process and reagent composition for determination of fructosamin Process and reagent composition for determination of fructosamin

1988-05-05

1991-10-08

Kepplinger, Ester L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 10, 435 14, 435 25, 435 28, 435269, 436 34, 436 63, 436 95, 436174, 436175, 436904, C12Q 100, C12Q 160, C12Q 126, G01N 100

Patent

active

050553887

ABSTRACT:
A process for the determination of fructosamine in body fluids by the reaction of a sample solution with a color reagent, wherein the sample liquid is mixed with a buffer solution having a pH value of from 9 to 12, a color-forming reagent and uricase, as well as with at least one detergent, and the chronological change of the extinction is measured kinetically in a temperature range of from 20.degree. to 40.degree. C. at the earliest after 5 minutes.

REFERENCES:
patent: 4282001 (1981-08-01), Klose et al.
patent: 4460684 (1984-07-01), Bauer
patent: 4642295 (1987-02-01), Baker
patent: 4645742 (1987-02-01), Baker
patent: 4649120 (1987-03-01), Steuer et al.
Johnson et al., "Fructosamine: A New Approach to the Estimation of Serum . . . ", Clin. Chim. Acta 127, 87-95 (1982).
Schleicher et al., "Specific Quantitation by HPLC of Protein (Lysine) Bound . . . ", J. Clin. Chem. Clin. Biochem. 19, 81-7 (1981).

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