Process and preparation for the quantitative determination of su

Details Process and preparation for the quantitative determination of su Process and preparation for the quantitative determination of su

1983-02-28

1985-07-09

Nucker, Christine M.

Chemistry of carbon compounds

Miscellaneous organic carbon compounds

C-metal

436503, 436504, 436545, 436547, 436804, 436815, C07G 700, G01N 3358, G01N 3360

Patent

active

045281319

ABSTRACT:
A process for preparing a stable receptor preparation suitable for the quantitative determination of substances able to bind to cerebral receptors in which a brain or brain-region material is homogenized with an aqueous solution of an inert substance soluble in water; the formed homogenizate is centrifuged at an acceleration of 800 to 110 g for 8 to 20 minutes to form a supernatant; the brain or brain-region material is isolated from the supernatant by centrifuging the supernatant at an acceleration of 18,000 to 22,000 g for 10 to 20 minutes, the thus-obtained solid substance is rehomogenized in distilled water; the homogenizate is frozen and then thawed and thereafter centrifuged at an acceleration of 7000 to 9000 g for 5-15 minutes; the supernatant is isolated, centrifuged at an acceleration of 35,000 to 45,000 g for 20 to 30 minutes; the obtained solid substance is washed with an aqueous buffer solution of a pH value between 6 and 8, and a suspension consisting of the solid substance and the washing liquid is frozen and then thawed at least once and thereafter the suspension is lyophilized.

REFERENCES:
Sigel et al., J. Biological Chemistry, 258 (1983), 6965-6971.
Enna et al., Chemical Abstracts, 84 (1976) #39778u.
Mackerer et al., J. Pharmacol. Exp. Ther., 206 (1978), 405-413.

Affiliated with

Also associated with

Rating

Process and preparation for the quantitative determination of su does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Process and preparation for the quantitative determination of su, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process and preparation for the quantitative determination of su will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-908453