Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1989-03-21
1990-07-03
Naff, David M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 22, 435 7021, 43524027, 4351722, 436518, 436548, 436824, 436828, 530387, 530808, 530809, 935106, 935110, G01N 33537, G01N 33541, G01N 33543, C12N 1500
Patent
active
049390821
ABSTRACT:
In body fluids containing saliva alpha amylase and pancreatic alpha amylase, pancreatic alpha amylase is determined with a monoclonal antibody which specifically binds but does not inhibit saliva alpha amylase and which has a cross-reactivity of 5% or less toward pancreatic alpha amylase. The monoclonal antibody binds salvia alpha amylase to form a complex which is separated to permit determining pancreatic alpha amylase with an amylase detection system. The complex may be separated by precipitating with a precipitating agent such as an anti-antibody or protein A, or by immobilizing the monoclonal antibody on a solid carrier. The monoclonal antibody is preferably produced by immunizing a host animal with an immunogen containing aluminum hydroxide, Bordatella pertussis and native saliva alpha amylase at least 7 times over a period of at least 9 months, fusing B lymphocytes of the immunized host animal with a transforming agent to produce hybridomas, cloning and culturing the hybridomas, and screening antibodies formed.
REFERENCES:
patent: 4012285 (1977-03-01), Pfleideier et al.
patent: 4474892 (1984-10-01), Murad et al.
patent: 4863728 (1989-09-01), Gerber et al.
Goding, J. W., Journal of Immunological Methods, 1980, vol. 39, pp. 285-308.
Mishell et al., Selected Methods In Cellular Immunology, W. H. Freeman and Co., San Francisco, 1980, pp. 43-54.
Whitlow et al., Clin. Chem., vol. 25, No. 3, 1979, pp. 481-483.
Hagele et al., Clin. Chem., vol. 28, No. 11, 1982, pp. 2201-2205.
Albert Winfried
Gerhardt Willie
Hubner-Parajsz Christa
Jungfer Herbert
Lenz Helmut
Boehringer Mannheim GmbH
Naff David M.
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