Process and monoclonal antibody for the specific determination o

Details Process and monoclonal antibody for the specific determination o Process and monoclonal antibody for the specific determination o

1990-10-09

1991-12-10

Naff, David M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 71, 435 76, 435 792, 435 22, 435 7021, 4351722, 43524026, 435975, 436518, 436548, 530808, 530809, 935106, 935108, G01N 33536, G01N 33543, C12Q 140, C12P 2108

Patent

active

050717448

ABSTRACT:
Pancreatic .alpha.-amylase is determined in body fluid in the presence of salivary .alpha.-amylase by combining the body fluid with a monoclonal antibody that inhibits salivary .alpha.-amylase by 95% or more and inhibits pancreatic .alpha.-amylase by 50% or less, and then detecting pancreatic .alpha.-amylase with a system for the detection of .alpha.-amylase. The monoclonal antibody may be in immobilized form. The monoclonal antibody is preferably produced by immunizing a Balb/c mouse or an AJ mouse with a specific immunogen for a specific number of times, fusing .beta.-lymphocytes obtained from the mouse with a myeloma cell line to form an antibody producing hybridoma cell line, cloning and screening the hybridoma cell line for the desired monoclonal antibody, and isolating the monoclonal antibody. The immunogen contains modified or unmodified salivary .alpha.-amylase, aluminum hydroxide and Bortadella pertussis.

REFERENCES:
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patent: 4939082 (1990-07-01), Naujoks et al.
patent: 4945043 (1990-07-01), Gerber
Patent Abstracts of Japan, Fractionation and Determination of Isozyme, vol. 8, No. 15 (C-206) (1452) 1/21/84, Sakata.
European Patent Applic 85300991.8, published Aug. 21, 1985.
Hagele et al., (1982) Clinical Chemistry, vol. 28, No. 11, pp. 2201-2205.
Derwent Publication Ltd., #C83118652 (1983).
Goding, (1980) Journal of Immunological Methods, vol. 39, pp. 285-308.
Whitlow et al., (1979) Clinical Chemistry, vol. 25, No. 3, pp. 481-483.
Chemical Abstracts (1984), vol. 100, No. 11, p. 241, item #82015d.

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