Process and medium for culturing ant venom gland cells

Details Process and medium for culturing ant venom gland cells Process and medium for culturing ant venom gland cells

1983-11-07

1985-11-19

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

435 41, 435948, 424 95, 424 98, C12N 500, C12P 100, C12R 191, A01N 6302, A61K 3558

Patent

active

045542511

ABSTRACT:
In vitro culture of the venom gland cells of the ant Pseudomyremex triplarinus is effected by removing venom gland tissue from a larva or pupa of P. triplarinus subjecting the tissue to a protease to separate out the cells therein and culturing the resultant cells in an isotonic saline medium comprising carbohydrate, amino acids guanosine 3',5'-cyclic monophosphate and adenosine 3',5'-cyclic monophosphoric acid, at least part of the medium having been conditioned with Spodoptera frugiperda cells. Such in vitro culture provides a practicable route to volume production of the biologically active materials which are useful for treating the symptoms of rheumatoid arthritis and which the venom gland cells are known to produce.

REFERENCES:
Miltenberger, H. G. (1983), Chem Abst. 100:118039f.
Marks, E. P. (1979), Chem Abst. 90:183456d.
Wilkinson, C. F. (1979), Chem Abst. 90:183457e.
Mitsuhashi, J. (1979), Chem Abst. 93:235655b.
Arthritis and Rheumatism, 25(4), Supplement Abstract C88, (Apr. 1982).
Proceedings of the North Sea Central Branch, Entomological Society of America 27, 153 (1972).
Chapter 28, Kursbak and Maramorosch (eds.), Invertebrate Tissue Culture, Academic Press (1976).

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