Process and medium for cloning and long-term serial cultivation

Details Process and medium for cloning and long-term serial cultivation Process and medium for cloning and long-term serial cultivation

1988-06-17

1991-02-19

Warren, Charles F.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

435 1, 43524023, 435948, A01N 102, C12N 500, C12N 100

Patent

active

049943871

ABSTRACT:
Vigorous growth of endothelial cells from human umbilical vein endothelial cells in vitro is achieved using a culture medium containing endothelial cell growth factor and heparin and/or dextran sulfate. Cell population doubling times of 13 to 21 hours for 60 to 85 populations doublings are obtained, and cloned human endothelial cell strains are established having similar proliferative capacities.

REFERENCES:
patent: 4443546 (1984-04-01), Stemerman et al.
patent: 4585654 (1986-04-01), Landaburn et al.
Thornton et al., "Human Endothelial Cells: Use of Heparin in Cloning and Long-Term Serial Cultivation", Science 222, pp. 623-625 (1983).
Yang et al., "The Effect of Heparin on Growth of Mammalian Cells in Vitro", Proceedings of the Society for Experimental Biology and Medicine 159, pp. 88-93 (1978).
Azizkhan et al., "Mast Cell Heparin Stimulates Migration of Capilary Endothelial Cells in Vitro", Journal of Experimental Medicine 152, pp. 931-944 (1980).
Roblin et al., "Cell Surface Changes Correlated with Density-Dependent Growth Inhibition, Glycosaminoglycan Metabolism in 3T3", Biochemistry 14(2), pp. 347-357 (1975).
Maciag, T. et al., Proc. Natl. Acad. Sci., U.S.A., 76:5674-5678 (1979), "An Endothelial Cell Growth Factor from Bovine Hypothalamus: Identification and Partial Characterization".
Maciag, T. et al., J. Cell. Biol. 91:420-426 (1981), "Serial Propagation of Human Endlthelial Cells In Vitro".
Glassberg, M. K. et al., In Vitro, vol. 18, No. 10, 859-865 (Oct. 1982), "Cultured Endothelial Cells Derived from the Human Iliac Arteries".
Laterra et al., "Functions for Fibronectin, Hyaluronate, and Heparan Proteoglycans in Substratum Adhesion of Fibroblasts", Extracellular Matrix ed., Hawkes et al., Academic Press 1982, pp. 197-207.

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