Process and medium for cloning and long-term serial cultivation

Details Process and medium for cloning and long-term serial cultivation Process and medium for cloning and long-term serial cultivation

1990-11-05

1992-07-21

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

43524023, 4352403, C12N 508, C12N 502

Patent

active

051322234

ABSTRACT:
Vigorous growth of endothelial cells from human blood vessels in vitro is achieved using a culture medium containing endothelial cell growth factor and heparin and/or dextran sulfate. Cell population doubling times of 13 to 21 hours for 60 to 85 populations doublings are obtained, and cloned human endothelial cell strains are established having similar proliferative capacities.

REFERENCES:
patent: 4994387 (1991-02-01), Levine et al.
Glassberg et al., In Vitro 18: 859-866 (1982).
Thornton et al., Science 222: 623-625 (1983).

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