Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Culture medium – per se
Patent
1997-07-15
1998-11-10
Lankford, Jr., Leon B.
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Culture medium, per se
435325, 435383, 435384, 435404, C12N 500
Patent
active
058343121
ABSTRACT:
Novel media and methods are disclosed for the in vitro formation of a histologically complete human epithelium. The media are serum-free, companion cell or feeder layer free and organotypic, matrix free solutions for the isolation and cultivation of clonally competent basal epithelial cells. The media and methods of the invention are useful in the production of epithelial tissues such as epidermis, cornea, gingiva and ureter.
REFERENCES:
patent: 4016036 (1977-04-01), Green et al.
patent: 4304866 (1981-12-01), Green et al.
patent: 4485096 (1984-11-01), Bell
patent: 4673649 (1987-06-01), Boyce et al.
patent: 4940666 (1990-07-01), Boyce et al.
patent: 5232848 (1993-08-01), Wolfe et al.
patent: 5326699 (1994-07-01), Torishima et al.
patent: 5328844 (1994-07-01), Moore
patent: 5686307 (1997-11-01), Wille, Jr.
Boisseau, et al., "Production of Epidermal Sheets in a Serum Free Culture System: A further Appraisal of the Role of Extracellular Calcium", J. of Dermatological Sci., 3, 111-120 (1992).
"Catalogue of Cell Lines & Hybridomas", American Type Culture Collection, 6th Ed., 342-343 (1988).
Coffey, et al., "Production and Auto-Induction of Transforming Growth Factor -.alpha. in Human Keratinocytes", Nature, 328, 817-820 (1987).
D. Peehl & R. Ham, "Growth and Differentiation of the Human Keratinocytes Without a Feeder Layer or Conditioned Medium", In Vitro, 16(6), 516-525 (1980).
G. Shipley & R. Ham, "Improved Medium and Culture Conditions for Clonal Growth With Minimal Serum Protein and For Enhanced Serum-Free Survival of Swiss 3T3 Cells", In Vitro, 17(8), 656-670 (1981).
H. Eagle, "Buffer Combinations for Mammalian Cell Culture", Science, 174, 500-503, (1971).
H. Green, "Cultured Cells for the Treatment of Disease", Sci. Amer., Nov. 1991, 96-102.
Judd, et al., "Culture of Human Keratinocytes in Defined Serum-Free Medium", Focus, 19(1), 2-5,(1977).
Nanchahal, et al., "Cultured Composite Skin Grafts: Biological Skin Equivalent Permitting Massive Expansion", The Lancet, Jul. 22, 191-193, (1989).
Nissley, et al., "Growth of Cells In Defined Environments: The Role of Endogenous Production of Insulin-Like Growth Factors", 337-344.
Pellegrini, et al., "Long-term Restoration of Damaged Corneal Surfaces With Autologous Cultivated Corneal epithelium", The Lancet, 349, 990-993, (1987).
S. Boyce & R. Ham., "Calcium-Regulated Differentiation of Normal Human Epidermal Keratinocytes in Chemically Defined Clonal Culture and Serum-Free Serial Culture", The J. of Inves. Derm., 81(1) Supp., 33s-40s (1983).
S. Hodson, "Cultivating a Cure for Blindness", Nature, 387, 449 (1997).
Tsao, et al., "Clonal Growth of Normal Human Epidermal Keratinocytes in a Defined Medium", J. Cellular Physi., 110, 219-229 (1982).
Wilke, et al., "Ability of Normal Human Keratinocytes That Grow in a Culture in Serum-Free Medium To Be Derived From Suprabasal Cells", J. of the Nat. Cancer Inst., 80(16), 1299-1304 (1988).
Wilke, et al., "Biologic Mechanisms for the Regulation of Normal Human Keratinocyte Proliferation and Differentiation", Am. J. of Pathology, 131(1), 170-181 (1988).
Wille, et al., "Effects of Growth Factors, Hormones, Bacterial Lipopolysaccharides, and Lipotechoic Acids on the Clonal Growth of Normal Ureteral Epithelial Cells in Serum-Free Culture", J. of Cellular Phys., 150, 52-58 (1992).
Wille, et al., "Integrated Control of Growth and Differentiation of Normal Human Prokeratinocytes Cultured in Serum-free Medium: Clonal Analyses, Growth Kinetics, and Cell Cycle Studies", J. of Cellular Physi., 121, 31-44 (1984).
Chopra, et al., "Propagation of Differentiating Normal Human Tracheobronchial Epithelial Cells in Serum-Free Medium", J. of Cellular Physi., 130, 173-181 (1987).
Shipley, et al., "Reversible Inhibition of Normal Human Prokeratinocyte Proliferation by Type .beta. Transforming Growth Factor-Growth Inhibitor in Serum-free Medium", Cancer Res., 46, 2068-2071 (1986).
Wille, et al., "Serum-Free Cultures of Normal Human Gingival Keratinocytes (HGK)", J. Derm. Res., 68, 1019 (1989).
Pittelkow, et al., "Two Functionally Distinct Classes of Growth Arrest States in Human Prokeratinocytes That Regulate Clonogenic Potential", The J. of Invest. Derm., 8(4), 410-417 (1986).
Hy-Gene, Inc.
Lankford , Jr. Leon B.
Nickey Donald O.
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