Liquid purification or separation – Processes – Utilizing electrical or wave energy directly applied to...
Reexamination Certificate
2002-08-19
2004-12-14
Lawrence, Frank M. (Department: 1724)
Liquid purification or separation
Processes
Utilizing electrical or wave energy directly applied to...
C210S764000, C422S024000, C422S186300, C119S226000
Reexamination Certificate
active
06830699
ABSTRACT:
The present invention relates to a method and a device for preventing blooms of micro-organisms in an aqueous system, and in particular for preventing blooms of toxin producing micro-algae and cyanobacteria.
In many countries, supply of drinking water is primarily based on the use of surface water, and lakes and rivers which are used as drinking water reservoirs are often vulnerable to blooms of toxin producing micro-algae and cyanobacteria. As a consequence of such blooms, the drinking water sources, which normally supply water of a high quality throughout the year, have had to close temporarily or completely.
Blooms of (micro-)algae also represent considerable ecological and economic problems in marine water systems.
In Norway, such blooms have caused mass deaths of farmed fish, and thus substantial economic losses for the industry. In other parts of the world, such blooms represent a direct threat to the human health situation, at the same time as large stocks of fish are poisoned.
To disinfect drinking water, it is common to treat the water chemically, for example by clorination. It is known that this has many side-effects. The treatment kills the micro-organisms but does not remove toxins which are already in the water. Furthermore, it is known to subject drinking water to UV-treatment in order to activate/kill micro organisms already present.
The principle which is the basis for the present invention is that algae and cyanobacteria are host organisms for viruses, and that an active virus population is often the reason for such algal- and bacterial blooms to culminate naturally.
In the USA and Israel, tests have been carried out in which one has used viruses to restrict blooms of harmful micro-algae. The procedure consists of isolating micro-algae and growing these to produce viruses. Thus, the viruses which are obtained will be specific to the population of algae they were originally collected from, and this specificity is also the likely reason for this procedure not having had the expected effect. In the time it has taken to cultivate the viruses in a monoculture of the algae, other algae will have bloomed. These new populations of algae will not be influenced by the produced viruses, and the effect is reduced or fails to appear.
To establish an effective procedure, the inventors of the present invention have used existing knowledge about UV-induction of viruses to establish a device and a method in which a fraction of a body of water is exposed to UV-treatment to induce virus populations, and in which the UV-treated fraction is thereafter returned to the rest of the water so that the viruses cause a culmination of, or limit the growth of the micro-organisms they are specific to. As the UV-treated fraction is returned to the water reservoir from which it came, and as this occurs immediately after the UV-treatment, the development of virus resistance will not be a problem. Furthermore, viruses will be developed against the different types of algae present in the body of water.
Thus, the present invention comprises a method and a device for treatment of a water system to limit populations of undesirable micro-organisms, or to kill micro-organisms, by inducing an activation of viruses that are latent in the micro-organisms mentioned.
The method according to the present invention is characterised in that a fraction of the aqueous system is subjected to a radiation treatment, and that said fraction is thereafter returned to the aqueous system.
The device, according to the present invention, is characterised in that it comprises a chamber in which a device is arranged to subject the water in the chamber to a radiation with a wavelength in the range 250-380 nm, and means to feed a quantity of water through the chamber.
Generally, it can be assumed that all organisms in nature will be exposed to attack by viruses. However, all viruses are host specific so that they only attack closely related organisms. The specificity is often associated with species, but the viruses can, in some cases, attack related species. The specificity can also be limited to one “strain” within one species.
Viruses occur in nature as free virus particles. These are inactive until they find a host which they can infect and multiply in. Whether a virus particle shall be taken up in a certain host, and whether the virus will be able to multiply in this host, and thereafter separate from the host cells to infect new hosts are based on complex biochemical mechanisms. It is this complex cooperation that makes the host-virus relationship to be very specific.
For algae and bacteria such a virus infection, and subsequent production of virus, will be lethal for the host micro-organisms. In such situations, the host-organisms will develop mechanisms to prevent such attacks, and subsequent virus activity. In higher organisms, the immune system of the organisms will, after some time, provide for identification and destruction of the virus particles. In lower order organisms, the evolution pressure will make the host organisms which develop a resistance (because of genetic multiplicity in such a host population) survive, and thus dominate the population. In this way, resistance to the original virus populations develops over a relatively short time. This leads in turn to the viruses having to be altered so that they again can infect the hosts. Thus, there will continuously be a evolution of both the host organisms and the viruses, and this is one of the reasons the procedure, in which algae are isolated for cultivation of a virus which is returned, does not work, as the time it takes to cultivate the viruses is sufficient for the host organisms to develop resistance, or new populations will have developed.
Furthermore, it is known from many systems (with the human system being one of them) that many organisms are carriers of non-active viruses, i.e. that the virus particles exist latent in the host. In such systems, the virus is under the control of the host so that it does not grow and cause damage. However, if the host is exposed to various kinds of external influences, for example, UV-radiation, then it could possibly lose control over the virus. The virus can then be activated, and it can multiply and can eventually kill the host organism. This will release virus particles which will attack other organisms of the same species.
Therefore, the principle for the invention according to the present application, is to manipulate the host-virus relationship by inducing an activation of the virus populations with the result being that the host organisms are killed, or restricted from growing and multiplying. According to the principle, all effective means which can be shown to lead to an increase in activity of the virus population can be used, but a preferred embodiment of the present invention relates to radiation with light, and especially light having a wavelength within the UV-range.
The following examples describe tests in mesocosmos scale in which the inventors of the present invention have measured the effect of UV-radiation on algae and viruses.
Without being restricted to a specific theory, it is assumed that the causal connection for the measured results is based on the above explanations of induction/activation of virus populations, with the subsequent decreases in algae counts. However, it shall not be excluded that other mechanisms can have had an effect on the measured result, for example, reduced production of toxins etc.
Due to the mentioned specific coupling between a virus and its host organism, there are no health risks associated with use of the procedures according to the present invention. The treatment will only kill or render harmless micro-organisms, and will not influence the health of the “end user”, be it fish in a marine environment, or people who drink the treated water. For the record, it shall be mentioned that freshwater normally contains between 1-10 million viruses per millilitre.
Seawater also contains normally between 1-10 million viruses per millilitre (Maranger, R. et al., Viral
Bratbak Gunnar
Heidal Mikal
Carella Byrne Bain et al.
Forinnova AS
Lawrence Frank M.
LandOfFree
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