Process and materials for producing soluble biological mediators

Details Process and materials for producing soluble biological mediators Process and materials for producing soluble biological mediators

1982-12-02

1986-05-27

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

435 2, 435 70, 435 91, 435240, 435241, 435811, 424101, C12P 2100, A01N 102, C12P 2102, C12P 1934, C12N 500, C12N 502, C01G 900

Patent

active

045915571

ABSTRACT:
A process and cellular system for producing soluble biological mediators, including cytokines, lymphokines, monokines and interferons, and mRNA therefor, from white blood cells is provided. The process involves treating white blood cells with a mitogenic or antigenic agent in the presence of a controlled amount of red blood cells. Increases in production of the soluble biological mediator, gamma interferon, on the order of 5 to 10 fold have been achieved by maintaining the ratio of red blood cells to white blood cells between 10 to 1 and about 50 to 1. The process is particularly useful in producing HuIFN-.gamma. with PHA-P.

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