Process and kit for isolating and purifying RNA from biological

Details Process and kit for isolating and purifying RNA from biological Process and kit for isolating and purifying RNA from biological

1991-07-10

1992-10-13

Lacey, David L.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, 435803, 536 27, 536 28, 536 29, 536127, C12P 1934, C12Q 168, C07H 1512, C07H 1700

Patent

active

051550180

ABSTRACT:
A process is provided for isolating and purifying biologically active RNA from a biological sources containing RNA, DNA and other cellular materials. The process involves contacting the RNA-containing source with particles comprising siliceous material, such as finely-divided glass, in the presence of a binding solution comprising concentrated, acidified chaotropic salt. Under these conditions, RNA, but not DNA, binds selectively to the siliceous material, and can be separated easily from the other components of the sample. Preferably, the selective binding process is applied to biological cells containing RNA of interest. Intact cells are disrupted by exposing them to a lysing solution comprising, as its main component, concentrated, acidified chaotropic salt. The RNA is then isolated and purified from the lysate using the selective binding process of the invention. The process of the invention may be conveniently provided as a kit, which may include the siliceous material, reagents and instructions for use of the kit to isolate and purify biologically active RNA from biological sources.

REFERENCES:
J. Chirgwin et al., Biochemistry, vol. 18, No. 24, pp. 5294-5298 (1979).
B. Vogelstein et al., Proc. Natl. Acad. Sci. USA, vol. 76, No. 2, pp. 614-619 (1979).
R. Boom et al., Journal of Clinical Microbiology, vol. 28, No. 3, pp. 495-503 (1990).

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