Process and kit for fragment cloning

Details Process and kit for fragment cloning Process and kit for fragment cloning

1998-11-17

2000-12-19

Yucel, Remy

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435243, 435260, 4353201, C12N 120

Patent

active

061626333

ABSTRACT:
The subject matter of the invention concerns three cosmid vectors which are suitable for fragment cloning of a size between 7 and 36 kb. These vectors consist of an E. coli ColE1 replica, an ampicillin resistance gene, a multiple cloning cassette, cos sites for in vitro packaging with the lambda packaging extracts as well as fragments from the genome of the bacteriophage lambda. The lambda sequences were selected in a way to prevent lytic processes, vector instabilities (deletions) and unwanted recombination events between lambda DNA and the fragment to be cloned. Depending on the length of the lambda fragment inserted in the corresponding vector heterologous fragments of different lengths can be cloned.

REFERENCES:
Methods In Enzymology 68, pp. 309-327, John Collins, Dec. 1979.
Hohn, B., and Collins, J. 1980, "A small cosmid for efficient cloning of large DNA fragments," Gene 11: 291-298.
Friedman, A.M. et al., 1982, "Construction of a broad host range cosmid cloning vector and its use in the genetic analysis of Rhizobium mutants," Gene 18: 289-296.
Knauf, V.C., and Nester, E.W. 1982, "Wide Host Range Cloning Vectors: A Cosmid Clone Bank of an Agrobacterium Ti Plasmid," Plasmid 8: 45-54.
Saito, I., and Stark, G.R. 1986, "Charomids: Cosmid vectors for efficient cloning and mapping of large or small restriction fragments," Proc. Natl. Acad. Sci. USA 83: 8664-8668.

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