Process and equipment for plasmid purification

Details Process and equipment for plasmid purification Process and equipment for plasmid purification

2006-04-11

2006-04-11

Wilson, James O. (Department: 1623)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C536S025300, C536S025310, C536S023100

Reexamination Certificate

active

07026468

ABSTRACT:
A scalable alkaline lysis process, including procedures and devices for the isolation of large quantities (grams and kilograms) of plasmid DNA from recombinantE. colicell. Effective, contgrollable, and economical operation, and consistent low level of host chromosomal DNA in the final plasmid product. Involves a series of new unit operations and devices for cell resuspension, cell lysis, and nuetralization.

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