Process and device for the separation and detection of component

Details Process and device for the separation and detection of component Process and device for the separation and detection of component

1995-10-31

1998-08-18

Arthur, Lisa B.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

935 77, 935 78, C12Q 168

Patent

active

057957207

ABSTRACT:
The invention relates to a process for the separation and detection of components of a mixture of materials by temperature gradient gel electrophoresis, wherein either

REFERENCES:
patent: 4671870 (1987-06-01), Tompa et al.
patent: 5066377 (1991-11-01), Rosenbaum et al.
Val C. Sheffield et al., "Attachment of a 40-base pair G+C-rich sequence (GC-clamp) to genomic DNA fragments by the polymerase chain . . . " Proc. Natl. Acad. Sci., USA, vol. 86, pp. 232-236, 1989.
Ann-Christine Sylvanen et al., "Quantification of polymerase chain reaction products by affinity-based hybrid collection", Nucleic Acids Research, vol. 16, No. 23, pp. 11327-11338, 1988.
Roger M. Wartell et al., "Detecting base pair substitutions in DNA fragments by temperature-gradient gel electrophoresis", Nucleic Acids Research, vol. 18, No. 9, pp. 2699-2705, 1990.
Richard M. Myers et al., "Nearly all single base substitutions in DNA fragments joined to a GC-clamp can be detected by . . . ", Nucleic Acids Research, vol. 13, No. 9, pp. 3131-3145, 1985.
Richard M. Myers et al., "Detection & Localization of Single Base Changes by Denaturing Gradient Gel Electrophoresis", Methods in Enzymology, vol. 155, pp. 501-527, 1987.
Detlev Riesner et al., "Temperature-gradient gel electrophoresis of nucleic acids: Analysis of conformational transitions, sequence variations, . . . ", Electrophoresis, vol. 10 (5-6), pp. 377-389, 1989.

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