Process and device for separating electrically charged macromole

Details Process and device for separating electrically charged macromole Process and device for separating electrically charged macromole

1989-11-14

1992-02-11

Niebling, John

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

204306, 204301, B01D 1302

Patent

active

050873385

ABSTRACT:
A process and apparatus for the continuous separation of electrically charged macromolecular compounds, such as proteins, by forced-flow membrane electrophoresis is provided.
Said process is conducted in an electrophoretic cell comprising a membrane compartment, divided by a micro- or ultrafiltration membrane into a feed and permeate cell, and electrode compartments being partitioned from the membrane compartment with ion-exchange membranes, which are impermeable to the macromolecular compounds, and have virtually no permeability to salts.
The electrophoretic process comprises the steps of recycling a mixed solution of the charged compounds which are dissolved in a separation buffer through the feed cell, subjecting of the solution to a pressure-driven membrane separation, applying an electric field across the membranes and the solution to cause electrophoretic migration of the charged compound and to separate them, and withdrawing the charged compounds thus separated.
The process achieves separation of charged compounds having differences of their isoelectric points in a range of as low as 0.1 to 0.2. The process is very useful to remove charged contaminants from the desired compounds.

REFERENCES:
patent: 3870617 (1975-05-01), Bourat
patent: 3972791 (1976-08-01), Stern
patent: 3989613 (1976-11-01), Gritzner
patent: 4043895 (1977-08-01), Gritzner
patent: 4146455 (1979-03-01), McRae
patent: 4673483 (1987-06-01), Mandle
patent: 4758320 (1988-07-01), Sanchez
patent: 4830720 (1989-05-01), Harada et al.

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