Process and device for producing bilayer vesicles

Plastic and nonmetallic article shaping or treating: processes – Encapsulating normally liquid material – Liquid encapsulation utilizing an emulsion or dispersion to...

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2103212, 424 37, 424 38, 424 43, 424 85, 424 88, 424172, 436829, B01J 1302, B01D 1300

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044380526

ABSTRACT:
A process for producing bilayer vesicles from a colloidal solution comprising mixed micelles of a bilayer-forming substance and a detergent, comprises removing the detergent from the micelle-containing colloidal solution by means of flow-through dialysis whereby the colloidal solution is dialyzed against a dialysis liquid in a chamber whose walls are at least partially formed by a semi-permeable membrane, wherein the dialysis liquid is moved along the outer side of the semi-permeable membrane at a velocity such that the detergent concentration in the dialysis liquid, on at least 90% of the active surface of the membrane, is at most 10% of the detergent concentration in the micelle solution in contact with the other side of the membrane, and wherein a homogeneous detergent concentration is maintained in the micelle solution by the movement of the latter.

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Biochemistry 18, 4173-4176 (1979).

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