Process and defined medium for growth of human epidermal keratin

Details Process and defined medium for growth of human epidermal keratin Process and defined medium for growth of human epidermal keratin

1986-12-09

1990-07-10

Weimar, Elizabeth C.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

43524023, 43524025, 4352403, C12N 500, C12N 502

Patent

active

049406663

ABSTRACT:
Disclosed are novel methods and materials for generating in vitro cultured populations of human epidermal keratinocyte cells having a characteristic colony-forming efficiency of greater than 20%. Novel media preparations and procedures are disclosed that permit isolation, serum-free primary culture and serum-free serial subculture of human epidermal keratinocytes. Also disclosed are procedures and products employing keratinocyte cells grown in serum-free conditions for medical application. i.e., skin grafting.

REFERENCES:
Tsao et al., "Clonal Growth of Normal Human Epidermal Keratinocytes in a Defined Medium", J. Cellular Physiology, vol. 110, .COPYRGT.1982; pp. 219-229.
Kruse et al. (eds.); Tissue Culture, Ch. 8 "Freezing, Storage, and Recovery of cell Stocks", pp. 712-718, .COPYRGT.1973.
Hawley-Nelson et al., "Optimized Conditions for the Growth of Human Epidermal Cells in Culture", J. of Invest. Derm., .COPYRGT.1980; pp. 176-182.

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