Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1983-07-15
1987-06-16
Hazel, Blondel
Chemistry: molecular biology and microbiology
Spore forming or isolating process
435241, C12N 500, C12N 502
Patent
active
046736492
ABSTRACT:
Disclosed are novel methods and materials for generating in vitro cultured populations of human epidermal keratinocyte cells having a characteristic colony-forming efficiency of greater than 20%. Novel media preparations and procedures are disclosed that permit isolation, serum-free primary culture and serum-free serial subculture of human epidermal keratinocytes. Also disclosed are procedures and products employing keratinocyte cells grown in serum-free conditions for medical application, i.e., skin grafting.
REFERENCES:
Tsao et al.; `Clonal Growth of Normal Human Epidermal Keratinocytes in a Defined Medium`, Cellular Physiology; vol. 110, .COPYRGT.1982; pp. 219-229.
Mariag et al., `An Endocrine Approach to the Control of Epidermal Growth-Serum-Free Cultivation of Human Keratinocytes`; Science, vol. 211; Mar. 27, 1981, pp. 1452-1454.
Tsao et al., "Clonal Growth of Normal Human Epidermal Keratinocytes in a Defined Medium", Chem. Abstr. #96:158772v (1982).
Hawley-Nelson et al., "Optimized Conditions for the Growth of Human Epidermal Cells in Culture", J. of Invest. Derm. (1980), pp. 176-182.
Kruse et al. (Eds.) "Tissue Culture", 1973, pp. 712-718.
Boyce Steven T.
Ham Richard G.
Hazel Blondel
University Patents Inc.
Weimar Elizabeth C.
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