Process and culture medium for the production of cells infected

Details Process and culture medium for the production of cells infected Process and culture medium for the production of cells infected

1996-11-20

2000-06-06

Minnifield, Nita

Chemistry: molecular biology and microbiology

Virus or bacteriophage, except for viral vector or...

43524021, 435239, 4242041, 4241841, 424 932, C12N 700, C12N 500, A61K 3712, A01N 6300

Patent

active

06071736&

ABSTRACT:
In a process for the in vitro production of a culture or cell line infected by a viral strain associated with multiple sclerosis (MS), a body sample is taken from an individual suffering from MS. The sample is cultivated in a culture medium that promotes the growth of infected cells to obtain a culture of primary infected cells. A sample of the culture of primary cells or a subculture of the latter is cultivated in series, by successive passages in the culture medium to obtain the culture or cell line infected by a virus associated with MS. The culture medium also contains a beta-interferon antibody or an antibody that is directed against an antigenically close molecule, the antibody playing an inhibiting role in viral expression and allowing long-lasting expression and propagation of the viral strain in the culture or cell line.

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