Process and composition for propagating mammalian cells

Details Process and composition for propagating mammalian cells Process and composition for propagating mammalian cells

1982-02-01

1984-04-17

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

435241, C12N 500, C12N 502

Patent

active

044435460

ABSTRACT:
A serum-free medium for use in propagating cells.
A process involving growing normal mammalian cells in vitro in a serum-free medium. The process includes providing the cells to be grown with various hormones as well as required nutrients in a serum-free environment.

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Uthne, "Large-Scale Production of Purified Somatomedin From Human Plasma," Acta Endocrinologica, pp. 12-35, (1973).
Hutchings, et al., "Growth and Maintenance of Hela Cells in Serum-Free Medium Supplemented with Hormones," Cell Biology, vol. 75, No. 2, pp. 901-904, Feb. 1978.
Bottenstein et al., "The Growth of Cells in Serum-Free Hormone-Supplemented Media," Methods in Enzymology, vol. LVIII, pp. 94-108, (1979).
Hayashi, "Replacement of Serum by Hormones Permits Growth of Cells in a Defined Medium," Nature, vol. 259, pp. 132-134, (1976).
Maciag, et al., "Hormonal Requirements of Baby Hamster Kidney Cells in Culture," Cell Biology International Reports, Jan. 1980.

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