Cutting – Processes – With subsequent handling
Patent
1995-02-14
1998-02-03
Rada, Rinaldi I.
Cutting
Processes
With subsequent handling
83 98, 83111, 83106, 83167, 839155, 83703, G01N 106
Patent
active
057132550
DESCRIPTION:
BRIEF SUMMARY
The invention relates to a process for the production of thin sections which, by means of a cutting blade of a microtome, are cut off a thin-section specimen held by means of a specimen holder of the microtome, and which are transferred to a liquid bath, and an apparatus for carrying out that process, which has a cutting blue and a specimen holder provided for a thin-section specimen from which a thin section is to be cut, the cutting blade and the specimen holder being movable relative to each other, a liquid bath being provided to receive the corresponding thin sections.
Such a process and an apparatus for carrying out the process are known from German patent specification No 22 00 415. In that process and apparatus the liquid bath directly adjoins the cutting edge of the cutting blade, with the liquid being in the rest condition; that however does not exclude the possibility of the individual thin sections suffering from a certain degree of buckling or upsetting, even if only relatively slight, in particular in the transitional region between the cutting edge of the cutting blade and the calm surface of the bath. In order to provide that the individual thin sections are transported away from the cutting blade, in an improved fashion in comparison with known microtomes which have a thin section-removal device of band-like nature, it is additionally necessary for the cutting blade to be provided with a cooling device and for the liquid container which contains the liquid to be provided with a heating device, in order to adjust the liquid to a given temperature. As the cooling device and the heating device the disposed relatively close together, not only does not that arrangement require suitable thermal insulation, but in addition it involves an amount of expenditure of energy for the cooling and heating devices, which is not negligible. A further disadvantage of that known apparatus is that the calm liquid bath receives not only the thin sections which are referred to as good thin sections, but also the unsatisfactory thin sections which are produced for example between those good thin sections and which represent wastage that cannot be put to use. That means however that operation with such a known microtome still leaves something to be desired.
An object of the present invention is to provide a process and an apparatus of the kind set forth in the opening part of this specification, wherein, while involving a low level of structure expenditure and using a comparatively small amount of energy, undesirable compression of the individual thin sections is avoided, and at the same time a section-stretching action is produced in a simple fashion, in respect of the individual thin sections.
In regard to the process, that object is attained in that the transfer of the thin sections from the cutting blade to the liquid bath is effected by a fluid flowing from the cutting blade to the liquid bath which is spaced from the cutting blade. The fluid which flows between the cutting blade or between the vicinity of the cutting edge of the cutting blade and the liquid bath produces, immediately adjoining the cutting operation, a transportation force which acts on the individual thin sections and with which said thin sections are transported away from the cutting edge of the cutting blade to the liquid bath, while at the same time the flowing fluid causes a stretching effect in respect of the individual thin sections. At the same time, that advantageously prevents undesired compression of the individual thin sections or corresponding portions thereof.
In that respect it has been found to be particularly advantageous if transfer of the thin sections from the cutting blade or from the cutting edge of the cutting blade of the microtome to the liquid bath is effected by means of a fluid which flows with a laminar flow. Such a laminar flow of said fluid produces an optimum stretching action in respect of the individual thin sections and consequently provides for avoiding compression thereof, so that undesirable buckling defor
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Izvozichikov Ilia Borisovitch
Mikhailov Serquei Petrovitch
Ashley Boyer
Microm Laborgerate GmbH
Rada Rinaldi I.
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