Process and apparatus for the genetic transformation of cells

Chemistry: molecular biology and microbiology – Plant cell or cell line – per se ; composition thereof;... – Plant cell or cell line – per se – contains exogenous or...

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435440, 435455, 435468, 435471, 435459, 4353201, 800278, 800295, 800300, C12N 1500, C12N 1529, C12N 1581, A01H 500

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active

058770232

ABSTRACT:
In a tubular pressure chamber a drop of a DNA solution with gold particles suspended therein is atomized at the opening of a cannula by a pressure impact. The fog droplets containing the gold particles and the DNA entrained by them, are pressured by the pressure impact through a restriction at the end of the pressure chamber and thereby accelerated and focused. They subsequently traverse in free flight an evacuated specimen chamber and impact in a narrowly limited target area with a predetermined pulse, cells fixed on a holder, thereby penetrating them.

REFERENCES:
patent: 4945050 (1990-07-01), Sanford et al.
patent: 5141131 (1992-08-01), Miller, Jr. et al.
patent: 5240842 (1993-08-01), Mets
"High-Velocity Microprojectiles For Delivering Nucleic Acids Into Living Cells", May 7, 1987 Nature, Klein et al, vol. 327, pp. 70-73.
"The Biolistic Process", Dec. 1988, Trends in Biotechnology, John Sandford, vol. 6, pp. 299-302.
"Factors Influencing Gene Delivery Into Zea Mays Cells By High-Velocity Microprojectiles", May 1988, Bio/Technology, T.M. Klein et al, vol. 6, pp. 559-563.
"Inheritance And Expression Of Foreign Genes In Transgenic Soybean Plants", Oct. 1989, Proceedings of the National Academy of Sciences in the USA, Paul Christou et al, vol. 86, No. 19, pp. 7500-7504.
"Transfer Of Foreign Genes Into Intact Maize Cells With High-Velocity Microprojectiles", Jun. 1988, Proc. Natl. Acad. Sci., Theodore Klein et al, vol. 85, pp. 4305-4309.
"Mitochondrial Transformation In Yeast By Bombardment With Microprojectiles", Jun. 1988, Science, Stephen Johnston et al, vol. 240, pp. 1538-1541.
"Transient Expression Of Foreign Genes In Rice, Wheat And Soybean Cells Following Particle Bombardment", 1988, Plant Molecular Biology, Yi-Chang Wang et al, vol. 11, pp. 433-439.
"Stable Transformation Of Soybean Callus By DNA-Coated Gold Particles", 1988, Plant Physiol, Christou et al, vol. 87, pp. 671-674.
"Delivery of Substances Into Cells And Tissues Using A Particle Bombardment Process",1987, Particulate Science and Technology, John Sanford et al, vol. 5, pp. 27-37.
"Shooting Genes Into Plant Cells", Jul. 1987, TibTech, Bryant, vol. 5, p. 181.
"DNA Delivery By Microprojectiles Accelerated By A `Vaccination Gun`", Apr. 1989, Miller et al, Journal of Cellular Biochemistry, vol. 13D, p. 264. Abstract M138.
"Chloroplast Transformation In Chlamydomonas With High Velocity Microprojectiles", Jun. 1988, Science, John Boynton et al, vol. 240, pp. 1534-1538.
"Stable Transformation Of Soybean (Glycine Max) By Particle Acceleration", Aug. 1988, Bio/Technology, Dennis E. McCabe et al, vol. 6, pp. 923-926.
"Transient Gene Expression in Maize, Rice, and Wheat Cells Using An Airgun Apparatus", 1990, Plant Physiol, James H. Oard et al, vol. 92, pp. 334-339.
"High Speed Beams of Small Particles", Gerhard W. Israel et al., Journal of Coloid and Interface Science 24, 330-337 (1967).
"Molecular Biology of The Cell", Alberts et al., 1983, Garland Publishing Inc., Title page and pgs. 11-12; 16; 20 and 1099.

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