Process and apparatus for recycling isoelectric focusing and iso

Details Process and apparatus for recycling isoelectric focusing and iso Process and apparatus for recycling isoelectric focusing and iso

1989-04-03

1990-01-30

Niebling, John F.

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

2041833, 204299R, G01N 2728, G01N 2726

Patent

active

048971693

ABSTRACT:
The disclosure is directed to a process and apparatus for isoelectric focusing and isotachophoresis of fluids. Recycling of the process fluid is established in a first direction through an electrophoretic chamber having parallel walls, the electric field being applied in a second direction. Externally of the chamber, the flow of fluid is subdivided into a plurality of closed loops, cooled to dissipate the Joule heat. While maintaining conditions of laminar flow within the chamber, unusual fluid stabilization against convective, electroosmotic and other fluid flow disturbances is obtained if the gap between the parallel walls of the chamber is kept narrow and the fluid flow is rapid, to maximize shear stress at the walls of the chamber and minimize the fluid residence time in the chamber.

REFERENCES:
patent: 3149060 (1964-09-01), Dorby et al.
patent: 3498905 (1970-03-01), Strickler
patent: 3519549 (1970-07-01), Grassmann et al.
patent: 4204929 (1980-05-01), Bier
patent: 4349429 (1982-09-01), Rhodes et al.
patent: 4362612 (1982-12-01), Bier
Cornelius F. Ivory et al., "Scale-Up of the Free Flow Electrophoresis Device", Electrophoresis, 83 (1983).

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