Process and apparatus for pulse labelling protein material in th

Details Process and apparatus for pulse labelling protein material in th Process and apparatus for pulse labelling protein material in th

1977-06-06

1979-05-08

Serwin, R. E.

Chemistry: physical processes

Physical processes

Crystallization

23230R, 2601125R, 422 67, 422 71, G01N 118, C07C10352

Patent

active

041534166

ABSTRACT:
A process and apparatus for pulse labelling protein material for high sensitivitiy sequence analysis of protein material by Edman degradation technique comprising an isolated, auxiliary unit for storing a radioactive coupling reagent together with tubes, valves and controls for interrupting the Edman process, for pumping a measure of radioactive coupling reagent into the reaction vessel, a timer for controlling duration of contact between the radioactive reagent, and the protein material to maximize the coupling reaction therebetween, and thereafter reactivating the Edman process to admit nonradioactive coupling reagent to the vessel and to drive the coupling reaction to completion.

REFERENCES:
patent: 3531258 (1970-09-01), Merrifield et al.
patent: 3557077 (1971-01-01), Brunfeldt et al.
patent: 3647390 (1972-03-01), Kubodera et al.
patent: 3725010 (1973-04-01), Penhasi

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