Process and apparatus for electrophoretic determination of prima

Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge

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204299R, G01N 2726

Patent

active

049591347

ABSTRACT:
An apparatus and process is provided for an electrophoretic determination the primary structure of nucleic acids. The apparatus comprises a cuvette shaped as a sleeve having a gel shaped as a hollow cylinder positioned on one of the side surfaces of the sleeve and having samples of the nucleic acid in recesses on its end face located in a circle. The sleeve is provided with a rotation drive and its side wall and the bottom together with the side wall and the bottom of the second sleeve mounted coaxially with the first sleeve form chambers for placing electrode solutions therein. Between the electrode solutions in the region between the side walls of the sleeves there is located a non-polar liquid, its density is higher than that of the electrode solution located above it and lower than the density of the electrode solution located thereunder. In the chambers annular electrodes are provided and a heat-exchanger in the non-polar liquid which maintains, through the non-polar liquid, the temperature of the gel shaped as a hollow cylinder at a predetermined level.

REFERENCES:
patent: 4624769 (1986-11-01), Simada et al.
patent: 4642169 (1987-02-01), Yoshisato et al.
patent: 4810183 (1989-03-01), Place et al.

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